NEBNextMultiplex Oligos for Illumina(Unique Dual Index UMI Adaptors DNA Set 4) are built for more streamlined and informative NGS multiplexing, tracking the sequencing reaction at the individual molecule level and enabling bioinformatic removal of misassigned reads for optimal accuracy. These index adaptors contain a pair of unique dual indices (i5 and i7) and one UMI sequence, making the resulting library multiplex-ready, with or without PCR. This set includes 96 unique dual index UMI adaptors, packaged in a single-use 96-well plate with a pierceable foil seal. The universal primer mix is supplied in a separate vial. Combining UMI Adaptors DNA Set 4 with one of the NEBNext DNA library prep kits is simple and straightforward if you follow the protocols found in the library prep kit manuals. With four sets of NEBNext Unique Dual Index UMI Adaptors available, you can multiplex up to 384 samples. Sample sheets for multiplexing are available.